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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be noticed in lots of tumors, which results in chromosome instability, and so may perhaps lead to tumorigenesis. This protein was discovered to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a significant position in marketing cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are actually explained. Two supplemental splice variants had been documented but in depth nucleotide sequence facts just isn't nevertheless obtainable.

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Blocking peptides are peptides that bind specially into the target antibody and block antibody binding. These peptide normally includes the epitope recognized via the antibody.

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This guidebook outlines additional feasible optimization of cellular and immunoassay parameters to be certain the very best benefits are...

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This manual outlines further more attainable optimization of cellular and immunoassay parameters to guarantee the best possible success are...

Antibodies bound to the blocking peptide no more bind to the epitope within the target protein. By evaluating the staining through the blocked antibody versus the antibody by itself, one can see which staining is unique.

Concentrate on Information and facts Cyclin E belongs into the really conserved cyclin spouse and children, whose customers are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation patterns which lead to the temporal coordination of each and every mitotic party. Cyclin E sorts a complex with and features being a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary and is also degraded as cells progress as a result of S period.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...

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